Fig. 6.

C9orf72 model zebrafish show adult onset swimming endurance deficits and reduced survival. (a) At nine months old, 2.2–7 transgenic zebrafish failed to continue swimming at earlier time points than their NTG clutch mates. N = 13 fish per genotype. (b) Also at twelve months old, 2.2–7 transgenic zebrafish failed to continue swimming at earlier time points than their NTG clutch mates. N = 13 fish per genotype. (c) Adult transgenic 2.2–7 zebrafish have reduced survival between 8 and 17 months in comparison to their NTG clutch mates which are housed in the same tank. N = 17 2.2–7 and 27 NTG at 8 months. (d) After being removed from the swim tunnel, 9 month old fish did not show any significant difference in proportion of transitions into slow (left), intermediate (middle) or fast (right) movements. N = 12 fish per genotype. (e) After being removed from the swim tunnel, 12 month old 2.2–7 zebrafish showed a significant increase in the proportion of transitions into slow movements and a corresponding significant decrease in the proportion of transitions into fast movement. There was no change in transition into intermediate movement. N = 13 fish per genotype. All data are shown as mean +/− standard deviation; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001