Table 2. Proteins exhibiting significant differences in abundance in LQ ejaculates as compared with HQ ejaculates (n = 6 in each group) (p < 0.05).

Up-arrows indicate protein spots with increased abundance in HQ ejaculates, while down-arrows indicate protein spots with increased abundance in LQ ejaculates.

Spot no.	Protein name	GI number	Sequence coverage(%)	Protein score	Ionscore > 30	Function in sperm cells	Cellularlocalisation	Ratio
1↑	Spermadhesin 1	157833803	81	123	2	Fertilisation	Cell surface(seminal plasma protein)	-4.2
4↑	Spermadhesin Z13	126158907	53	389	3	Fertilisation	Cell surface(seminal plasma protein)	-2.8
2↑	Binder of sperm protein 1	20663780	52	349	2	Capacitation	Cell surface(seminal plasma protein)	-2.1
3↑	Binder of sperm protein 1	20663780	21	148	1	Capacitation	Cell surface(seminal plasma protein)	-2.0
9↑	Binder of sperm protein 5	28849953	19	229	3	Capacitation	Cell surface(seminal plasma protein)	-4.0
10↑	Binder of sperm protein 5	28849953	24	148	2	Capacitation	Cell surface (seminal plasma protein)	-3.4
12↓	Cytochrome b-c1 complex, mitochondrial	3891848	54	281	2	Energetic metabolism	Mitochondria	1.2
13↓	Cytochrome b-c1 complex, mitochondrial	3891848	43	324	4	Energetic metabolism	Mitochondria	1.2
8↑	Adenylate kinase isoenzyme 1	61888850	68	207	2	Energetic metabolism	Cytoskeleton	-4.6
11↑	Succinyl-CoA ligase subunit beta, mitochondrial	77736229	36	276	2	Energetic metabolism	Mitochondria	-1.2
14↓	L-lactate dehydrogenase C isoform 1	296471858	61	278	2	Energetic metabolism	Mitochondria	1.3
						Fertilisation	Plasma membrane
							Cytoplasm
5↑	Superoxide dismutase	88853816	39	240	2	Cellular detoxification	Mitochondria	-6.3
							Cytoplasm
							Extracellular fluid
6↑	Glutathione S-transferase mu 1	114053087	80	723	7	Cellular detoxification	Plasma membrane	-5.5
						Fertilisation
7↑	Glutathione S-transferase mu 1	114053087	40	343	3	Cellular detoxification	Plasma membrane	-6.1
						Fertilisation