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. 2018 Nov 14;13(11):e0207227. doi: 10.1371/journal.pone.0207227

Fig 1. Steady-state levels of colon cancer cell lines.

Fig 1

Using Western blot assay steady protein levels of pEGFR, autophagy markers LC3B, and PD-L1 analyzed in basic colon adenocarcinoma cell lines (Caco-2, RKO, colo-205 and HCT116). As a control cell, line Caco-2 is used as an intermediate adenoma cell line. The quantification of LC3 reflects the ratio of LC3II/LC3I in comparison with Caco-2 in each sample separately. Each cell line was compared with Caco-2 for the protein levels of pEGFR, autophagy levels and the presence or absence of PD-L1 protein levels.