Figure 4.

SE1 suppressed PMA-induced JNK, and NF-κB activation in HT1080 cell. (a) Cells were pretreated with different concentrations (10, 50, and 100 μM) of SE1 for 1 h and PMA (10 ng mL⁻¹) incubation was continued for 24 h. Total cell lysates were evaluated for MAPK phosphorylation/degradation using western blotting. Band intensities were normalized to β-actin expression, and then the relative ratios of phosphorylated form/total form were calculated. (b) The effect of SE1 on the nuclear translocation of the NF-κBp65 subunit under PMA stimulation was examined. β-actin was used as loading controls. The results presented are the means ± S.D. of a triplicate experiment. ^#P < 0.001, ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, PMA stimulation.