Figure 5.

Mutants with Altered Sensitivity to ES4.

(A) Quantification of root growth sensitivity of the wild type (Ws, Ler, and Col-0), mutants (rcn1, van7, gnom^R5, axr2-1, axr1-12, chc1-2, snx1-1, vti12, chc2-2, big3, ben1-1, ben1-2, gnl1-3, and gnl1-2), and overexpression lines (35S_pro:PID21 and 35S_pro:PIN1). Seedlings were grown for 7 d on growth medium supplemented with mock or 17 µM ES4. Ratio of root lengths grown on ES4 to those grown on mock was calculated. Values represent means ± se of three independent experiments, each consisting of 5 to 32 analyzed roots per treatment for each line. Asterisks indicate significant difference between mutant and corresponding wild type (two-tailed Student’s t test, **P < 0.01 and ***P < 0.001).

(B) Representative images of the ES4 sensitivity of gnl1 mutants manifested by a highly reduced root length compared with the control. Wild-type, gnl1-2, and gnl1-3 seedlings were grown for 7 d on growth medium supplemented with 17 µM ES4. Bars = 10 µm.