Figure 8.

DARTS-Based Validation of the Association of ES4 with Arabidopsis ARF-GEFs.

(A) DARTS analysis of the susceptibility of protein to proteolytic degradation in the presence of 250 µM BFA or 250 µM ES4. Total protein extracts from Arabidopsis PSB-D cell cultures were incubated with the respective compound and then challenged with different dilutions of protease. The ARF-GEF protein levels were detected through protein gel blot analysis. ATPβ was used as reference protein control.

(B) Quantification of the protein band intensity reveals that the presence of ES4 stabilizes all Arabidopsis ARF-GEFs with exception of GNL1 and GNL2. Values represent means ± se of representative immunoblots from three biological repeats. Asterisks indicate significant difference between BFA (or ES4) and DMSO treatments (two-tailed Student’s t test, ***P < 0.001, **P < 0.01, and *P < 0.05).