Table 2. T6P Binding to KIN10 Weakens the Interaction between GRIK1and KIN10.
| Target | Ligand(s) | Kd (µM) |
|---|---|---|
| KIN10 | ATP | 12.8 ± 1.1 |
| KIN10 | T6P | 32.0 ± 5.6 |
| GRIK1 | T6P | – |
| KIN10 | GRIK1 | 19.5 ± 2.6 |
| KIN10 | ATP+T6P (50 µM) | 12.9 ± 1.2 |
| KIN10 | GRIK1+T6P (50 µM) | 67.7 ± 9.8 |
MST was performed to determine the equilibrium dissociation constants (Kd) between KIN10, GRIK1, and T6P. Values represent mean ± sd, n = 3 independent replicates. Kd values for KIN10 and GRIK1 in the presence of 50 μM T6P were significantly different (P < 0.002 by Student’s t test of three independent replicates) from those in the absence of T6P. –, Lack of observed interaction between GRIK1 and T6P.