Figure 8.

Oxalate Contents and Metabolome Analysis in the Wild Type and ocd1.

(A) Measurement of oxalate contents in mature wild-type, W22, o7, and ocd1-1 seeds. Error bars represent ±sd from three biological replicates.

(B) Significant fold changes of metabolites in wild-type and ocd1-1 20-DAP endosperms revealed by targeted metabolomics analyses.

(C) Principal component analysis of nontargeted metabolomes. Metabolites were extracted from six individual endosperms at 20 DAP and examined under positive and negative mode.

(D) Relative contents of variant saccharides with significant fold changes in ocd1-1 compared to the wild type.

(E) Relative contents of variant amino acids with significant fold changes in ocd1-1 compared to the wild type.

(F) Relative contents of taurine and hypotaurine in the wild type and ocd1-1.

(G) Concentrations of ATP in the wild type and ocd1-1. Levels of ATP were quantified enzymatically.

(H) Relative content of indole acetic acid in the wild type and ocd1-1.

Levels of metabolites (except for ATP) in wild type were designated 1. The error bars indicate ±sd from six biological replicates.