Figure 12.
SCL14-Dependent Detoxification Pathway Acts on Toxic RCS Rather Than on ROS Accumulation.
(A) Leaf bleaching (top panel) and lipid peroxidation, monitored by autoluminescence imaging (bottom panel), of wild-type plants pretreated with 1 mM HNE or with water. The color palette shows signal intensity from low (dark blue) to high (white) values.
(B) HPLC-UV quantification of the HOTE normalized to the wild-type control (CTRL). The black diamonds indicate significant differences with P < 0.05. Error bar = +sd between the four biological replicates from leaves of three plants per treatment.
(C) SOSG or H2DCFDA fluorescence in wild-type, scl14, or OE:SCL14 plants tested measured after 4 h of high-light stress. Error bars = +sd between fluorescence deriving from five leaves per plant. Two plants per genotype per treatment.