Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 10;30(10):2383–2401. doi: 10.1105/tpc.17.00586

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 American Society of Plant Biologists. All rights reserved.

PMC Copyright notice

Figure 8. — CIKs Physically Interact with BAM1/2 and RPK2.

(A) mbSUS yeast two-hybrid results showing that CIKs interact with BAM1/2 and RPK2. SC, synthetic complete medium; SD, synthetic minimal medium. pMetYC/pX-NubWT, negative control; N-KAT1/KAT1-Cub, positive control. ERL2 was used as a negative control.

(B) BiFC results showing that CIKs interact with BAM1/2 and RPK2 in epidermal cells of tobacco leaves. BRI1-YN/BAK1-YC, positive control; YN/YC, negative control. ERL2 was used as a negative control. Bar = 20 µm.

(C) to (E) Coimmunoprecipitation analyses showing that CIKs interact with BAM1/2 and RPK2 in vivo. Total proteins were extracted from leaves of 3-week-old double transgenic plants expressing GFP-tagged BAMs, RPK2, or FLS2 and CIKs-FLAG. Proteins immunoprecipitated with an α-Flag antibody were analyzed with an α-GFP antibody (IP, α-Flag; IB, α-GFP) or an α-Flag antibody (IP, α-Flag; IB, α-Flag). Protein extracts before immunoprecipitation were analyzed with an α-GFP antibody (IB, α-GFP) or an α-Flag antibody (IB, α-Flag) as input controls. FLS2-GFP CIKs-FLAG double transgenic plants and BAM1-GFP, BAM2-GFP, or RPK2-GFP single transgenic plants were used as negative controls. These experiments were repeated at least three times independently with similar results.