Figure 10.

Impact of Truncated VTI11 Mutant on TuMV Intercellular Movement.

(A) Expression of VTI11^ΔTMD:CFP in N. benthamiana as observed by confocal microscopy with the 10× objective (bigger panel) and 63× objective (inset) during intercellular TuMV movement. Bars = 10 μm in insets and 1 mm in main panels.

(B) VTI11^ΔTMD:CFP was transiently expressed 1 d before TuMV/6K₂:mCherry//GFP-HDEL agroinoculation. Expression of GFP and mCherry fusions was acquired at 5 dpi by confocal microscopy. Panels are representative images of infection foci corresponding to the median value for the indicated treatments. Data sets for mock and SYT truncated mutants treatments are graphically presented as whisker and box plots. Statistical significance was tested with the nonparametric Wilcoxon-Mann-Whitney two-sample rank test (*P < 0.05). n represents the number of infection foci analyzed.

(C) Viral replication was evaluated by RT-qPCR at 3.25 dpi from N. benthamiana agroinfiltrated with pCambia (Buffer), pCambiaTuMV^VNN (VNN), or pCambiaTuMV (TuMV) 1 d after pCambia (mock) or pCambiaVTI11^ΔTMD:CFP infiltration. Error bars show the sd calculated from values obtained in the three biological replicates. Three independent biological repeats were performed for (A) to (C).