Figure 11.

Viral RNA is Associated with Multivesicular Bodies.

(A) and (B) Immunogold labeling was performed on mock- and TuMV-infected N. benthamiana leaf tissue cross sections using an anti-dsRNA specific antibody. Transmission electron microscopy representative images show the presence of gold particles in the mock-infected condition (A) and in TuMV-infected cells (B). Three independent biological replicates were performed. Bars = 500 nm.

(C) The density of gold particles in MVBs from mock-infected versus TuMV-infected cells is shown. Two different labeling experiments were considered for quantification, and 200 gold particles were counted for each experiment. Error bars show the sd calculated from values obtained in two biological replicates. Chl, chloroplast; CI, cytoplasmic inclusion body; Cyt, cytosol; CW, cell wall; DMV, double-membrane vesicle; M, mitochondria; Vac, vacuole.