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. Author manuscript; available in PMC: 2019 Feb 12.
Published in final edited form as: Int Rev Cell Mol Biol. 2018 Feb 12;337:83–110. doi: 10.1016/bs.ircmb.2017.12.007

Figure 2: LD Biogenesis.

Figure 2:

COS-1 cells were transfected to express a model peptide consisting of the hydrophobic domain of the methyl transferase ALDI, fused to the LD-targeting signal of caveolin. This model peptide is initially targeted to the ER, and accumulates in microdomains where LD biogenesis will occur. Transfected cells were treated with oleic acid and a fluorescent fatty acid analogue (FA-BODIPY). During LD biogenesis, the FA-BODIPY accumulated in an ER microdomain, forming a nascent LD. Numbers indicate time in minutes after the addition of oleic acid and FA-BODIPY. Modified with permission from Kassan et al., 2013.