Examples of LD-organelle contacts in segmented multispectral lattice light-sheet images. Shown are contacts between LDs (blue) and ER (yellow), mitochondria (green), Golgi (red), lysosomes (magenta), or peroxisomes (cyan). Scale bar, 2 μm. Image credit: Sarah Cohen, Alex Valm, Wesley Legant, Eric Betzig, and Jennifer Lippincott-Schwartz. Images acquired as described in (Valm et al., 2017).