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Immunohistochemical staining of type I collagen showed increase from 2 weeks to 20 weeks around the 45° crack but no presentation around the 90° crack. (a) 45° crack at 2 weeks. (b) 45° crack at 6 weeks. (c) 45° crack at 20 weeks. (d) 90° crack at 2 weeks. (e) 90° crack at 6 weeks. (f) 90° crack at 20 weeks. Scale bar: 200 μm.
