Table 2.
Calculated EC50 values for the contractile and relaxation response.
| Vessel | Agonist | EC50 (mean ± SEM)
|
||||||
|---|---|---|---|---|---|---|---|---|
| naïve | PVP | 20 nm AgNP/PVP | 110 nm AgNP/PVP | citrate | 20 nm AgNP/citrate | 110 nm AgNP/citrate | ||
| MUA |
PE (nM) |
269.8 ± 135.0 | 538.1 ± 62.4 | 491.9 ± 115.4 | 362.9 ± 141.4 | 607.1 ± 41.3 | 522.7 ± 69.2 | 604.6 ± 42.5 |
|
ET-1 (nM) |
2.2 ± 0.9 | 4.4 ± 1.5 | 2.8 ± 1.5 | 1.4 ± 0.8 | 3.5 ± 0.6 | 4.0 ± 1.2 | 5.7 ± 2.1 | |
|
ANGII (nM) |
2.5 ± 0.7 | 2.2 ± 0.5 | 1.3 ± 0.4 | 0.6 ± 0.1 | 1.0 ± 0.2 | 2.6 ± 0.6 | 2.0 ± 0.5 | |
|
Ach (nM) |
211.7 ± 102.1 | 458.1 ± 85.7 | 215.6 ± 130.9 | 196.0 ± 80.5 | 351.4 ± 59.7 | 304.4 ± 38.6 | 333.7 ± 94.9 | |
|
| ||||||||
| MA |
PE (nM) |
1564.0 ± 332.0 | 1511.0 ± 205.5 | 1370.0 ± 705.4 | 694.5 ± 58.5* | 1328.0 ± 728.0 | 788.5 ± 118.5 | 1183.0 ± 269.6 |
|
ET-1 (nM) |
5.3 ± 1.3 | 4.3 ± 2.5 | 4.4 ± 2.1 | 1.4 ± 0.3 | 2.3 ± 1.4 | 1.6 ± 0.2 | 2.9 ± 0.4 | |
|
5HT (μM) |
270.6 ± 79.4 | 291.4 ± 22.7 | 284.8 ± 13.5 | 261.6 ± 24.4 | ND | 443.4 ± 104.9 | 281.5 ± 26.3 | |
|
Ach (μM) |
38.4 ±11.8 | 220.5 ± 14.5# | 59.0 ± 53.1* | 47.9 ± 29.7* | 57.5 ± 18.5 | 120.0 ± 12.2 | 167.5 ± 80.9 | |
|
| ||||||||
| TA |
PE (μM) |
125.1 ± 25.8 | 71.6 ± 12.0 | 54.2 ± 19.1## | 146.1± 31.6* | 104.4 ± 22.4 | 138.4 ± 25.3† | 99.1 ± 11.2 |
|
Ach (nM) |
49.4 ± 11.8 | 3226. 0 ± 522.3# | 972.9 ± 792.4* | 263.4 ± 209.0* | 152.2 ± 71.3 | 560.4 ± 129.3# | 1486.0 ± 1414.0 | |
EC50 values for cumulative concentration responses reported in Figures 2–6 were calculated using the Hill equation. MUA: main uterine artery, MA: mesenteric artery, TA: thoracic aorta, PE: phenylephrine, ET-1: endothelin 1, ANG II: angiotensin II, Ach: acetylcholine and 5HT: serotonin.
*
indicates p < 0.05 compared to stabilization vehicle (PVP or citrate),
#
indicates p < 0.05 compared to naïve,
##
indicates p < 0.05 compared between 20 and 110 AgNP in the same stabilization vehicle and
†
indicates p < 0.05 compared between PVP and citrate formulations of the same size AgNP, using a two tailed t test (n = 3 – 10).
ND = not determined