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. Author manuscript; available in PMC: 2019 Mar 20.
Published in final edited form as: ACS Macro Lett. 2018 Feb 20;7(3):324–329. doi: 10.1021/acsmacrolett.7b00974

Figure 4.

Figure 4

Characterization of insulin macroinitiator and insulin-trehalose glycopolymer conjugate. (A) Analytical HPLC and (B) MALDI MS after purification of the insulin macroinitiator, (C) Coomassie stained SDS PAGE (Lane 1: Ladder, Lane 2: Insulin, Lane 3: Purified insulin macroinitiator, Lane 4: Trehalose glycopolymer, Lane 5: Crude insulin-trehalose glycopolymer conjugate, Lane 6: Purified insulin-trehalose glycopolymer conjugate), (D) native PAGE with Western blot analysis (Lane 1: Insulin, Lane 2: Purified insulin macroinitiator, Lane 3: Trehalose glycopolymer, Lane 4: Crude insulin-trehalose glycopolymer conjugate, Lane 5: Purified insulin-trehalose glycopolymer conjugate Lane 6: Insulin-trehalose glycopolymer conjugate after digestion with Proteinase K), and (E) SEC trace of trehalose glycopolymer after digestion of insulin with Proteinase K.