Table 7.
In silico prediction of TLR2 nucleotide variation on splicing phenotype
| TLR2 SNP | Point mutation | Sliceman | MutationTaster | |||||
|---|---|---|---|---|---|---|---|---|
| Wildtype (wt) | Mutation (mt) | L1 distance (AU) | Ranking (L1) (%) | Effect | Score | wt detection sequence | ||
| rs3804099 | cagaa(c/t)gtaag | aacgta | aatgta | 35,539 | 70 | Donor increased | wt: 0.26/mu: 0.41 | wt: CAGAATGTAAGTCAT mu: CAGAACGTAAGTCAT |
| rs3804100 | cacag(c/t)gtaac | cagcgt | cagtgt | 34,758 | 66 | Donor gained | 0.35 | mu: GAATACACAGCGTAA |
| rs4696480 | tctgg(a/t)gaggg | ctggag | ctggtg | 32,723 | 56 | VNA | VNA | VNA |
Note: L1: distance to its corresponding bin (ie, a comparison between two hexamers that resulted in low L1 distance would be assigned with a low percentile rank).
Abbreviations: VNA, applicable where the values were not obtained; SNP, single-nucleotide polymorphism.