Figure 1.

Positive effect of FSH on subcutaneous tumors in vivo and in vitro.

Notes: Nude mice were injected with HeLa cells for 21 days, accompanied by 20 mIU/mL FSH or in the absence of FSH before/after tumor occurred, and HeLa cells were untreated or treated with 20 mIU/mL FSH for 60 hours. (A) The effect of different FSH concentrations on the cell number of HeLa cells (n=8). (B) The occurrence of tumor mass and the weight of tumor mass in nude mice with the different treatments (n=12). (C) The number of HeLa cells untreated or treated with FSH using the MTT assay (n=8). (D) The detection of cell cycle and apoptosis by flow cytometry (n=3). (E) Western blotting levels of cleaved CASP3 level in HeLa cells that were untreated or treated with FSH. Data are mean ± SEM (n=6). ^*P<0.05, ^**P<0.01.

Abbreviations: FSH, follicle-stimulating hormone; CASP3, Caspase 3.