Fig. 4.

The effect of MSC-CM on key proteins in the PAF/PAFR pathway in OCCs. As described in our previous study, key proteins involved in the PAF/PAFR pathway, cyclin D1 and phosphorylated FAK, were induced by MSC-CM. For the immunofluorescence staining of phosphorylated FAK (a) and cyclin D1 (b) in SKOV3 cells, after 24 h of incubation with MSC-CM or PAF with or without GB, the cells were labelled with polyclonal antibodies against cyclin D1 and phosphorylated FAK overnight and then incubated with a fluorescent secondary antibody for 1 h and stained with DAPI for 10 min (with magnification 40×). MSC-CM induced cyclin D1 expression and FAK phosphorylation, and these effects were blocked by GB. These effects were confirmed by Western Blot analysis (c, d and e)