Figure 4.

Apoptosis analysis.

Notes: (A) Cells were stained with annexin V-FITC/PI and analyzed by flow cytometry. Cells were incubated with BU, BU@VeC micelles, BU@VeC/T MM, or BU@VeC/T-RGD MM for 48 hours in LoVo/ADR cells. Untreated cells served as control and (B) column chart showing the average of three studies, ^*P<0.05 and ^**P<0.01 indicate statistical significance levels compared with the control. (C) Western blot analysis of apoptosis-related proteins expression in LoVo/ADR.

Abbreviations: BU, bufalin; MM, mixed micelles.