Figure 5.

Effect of BU-loaded micelles on accumulation of Rho123 and the protein expression of P-gp.

Notes: (A) Fluorescence intensity of LoVo/ADR cells after 1 hour of incubation with the free Rho123 (5 µM) in the absence and presence of different formulations. (B) The relative values of MFI in the absence or presence of Rho123-labeled micelles were measured by flow cytometry in LoVo/ADR. The results are presented as fold change in fluorescence intensity relative to control. (C) Western blot analysis of P-gp. Effect of BU-loaded micelles on the protein expression of P-gp in LoVo/ADR cells. (D) Quantitative analysis of effects of different groups on P-gp expression. ^*P<0.05, ^**P<0.01, and ^***P<0.001 indicate statistical significance levels compared with the control.

Abbreviations: BU, bufalin; MFI, mean fluorescence intensity.