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. 2017 Dec 12;29(1):29–34. doi: 10.1021/acs.bioconjchem.7b00675

Figure 1.

Figure 1

Dual labeling strategies based on protected (disulfide) and nonprotected thiols. (A) Sequential labelling of free thiol with maleimide followed by disulfide rebridging by allyl-sulfone reagent. (B) Maleimide-based sequential labeling introducing two new reagents at distinct sites.