Figure 11.

(a-c) Typical I-t traces recorded at −120 mV for the translocation of 9-, 17-, and 60-bp dsDNA, with an expanded view of the end of the event. Only successful translocation events are analyzed. Additional examples for each dsDNA are shown in Figure S18-S20. Duration histograms of $t_{\mathrm{M}}$ and $t_{\mathrm{D}}$ are fitted by exponential decay and exponentially modified Gaussians (red curves), respectively. The most probable translocation times $t_{\mathrm{p}}$ are obtained from the fitted curves and plotted in (d) for applied potentials of$$−100, −120, and −140 mV. Note that the 60-bp dsDNA did not translocate through the protein channel at the lowest applied potential, i.e., −100 mV. The y-axis error bars were obtained from 3 or 4 experiments. The data were collected in 3 M KCl, 20 mM HOAc/KOAc, pH 5.0, using a 10-kHz Bessel filter (100-kHz Bessel filter for 9-bp data) and post-filtered at 3 kHz for presentation.