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. Author manuscript; available in PMC: 2019 Oct 31.
Published in final edited form as: J Am Chem Soc. 2018 Oct 16;140(43):14224–14234. doi: 10.1021/jacs.8b08153

Figure 3.

Figure 3.

Single-channel I-t traces (5 s) obtained in 1, 2, and 3 M KCl at applied potentials of −50, −80, and −120 mV (cis vs trans). When the channel transits to a closed state and does not recover to the open state, a reversal potential (+50, +80, +120 mV) is applied as indicated by the arrow. Experiments were carried out at 20.0 °C in pH 5.0 solutions. The I-t traces were post-filtered at 2 kHz for presentation.