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. Author manuscript; available in PMC: 2019 Oct 31.
Published in final edited form as: J Am Chem Soc. 2018 Oct 16;140(43):14224–14234. doi: 10.1021/jacs.8b08153

Figure 6.

Figure 6.

Capture and release of dsDNA using the γ-HL nanopore. (a) I-t trace in the absence of DNA (left) and in the presence of 2 μM 60-mer ssDNA in the cis chamber (right). (b) I-t traces corresponding to 30-bp dsDNA molecules with a poly(dA)30 tail (sequence shown on the top). The long blockades (> 100 ms) display modulation between a mid-level blockade (IM) of ~40% and a deeper blockade (~20%). (c) I-t traces for two types of dsDNA blockade events. Type 1 events terminate from IM to the open-channel current I0 directly. Type 2 events exhibit a deep-blockade current of ~5% at the end before returning to the open channel current. Data were filtered by a Bessel filter at 100 kHz for (a) and 10 kHz for (b)-(c). The I-t traces were post-filtered at 10 kHz (a) and 2 kHz for presentation (b-c), respectively.