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(A) Western blot analysis of WT and Klf5-KO ESCs. Western blot analysis was performed with antibodies against Klf2, Klf4, and β-actin. (B) Quantified signal intensity of Klf2 or Klf4 relative to β-actin. (C) RT-qPCR analysis of Klf2 and Klf4 in WT and Klf5-KO ESCs.
