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. 2018 Nov 19;13(11):e0207321. doi: 10.1371/journal.pone.0207321

Fig 6. Chimera-forming ability of EpiSCs reprogrammed by Klf5 with Chiron/LIF.

Fig 6

Reprogrammed Epi-iPSCs show the hallmarks of naïve pluripotency. (A) RT-qPCR analysis of expression of markers for naive pluripotency and differentiation. After the Klf5 expression cassette was removed by transient Cre expression, RT-qPCR analysis was performed. Levels of expression relative to those in ESCs are shown. (B) Chimera generated from Epi-iPSCs induced by Klf5 in combination with Chiron. Upper panels show a control WT embryo and embryo injected with GFP-labeled Epi-iPSCs on the left and right, respectively. Lower panel shows a postnatal chimera at P10 injected with GFP-labeled Epi-iPSCs. Note that brown coat color indicates the contribution of 129 Epi-iPSCs. Plotted results are mean and SEM of three independent experiments. Scale bar: 100 μm.