REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bacterial and Virus Strains
E. coli Rosetta 2 (DE3)	EMD Millipore	Cat#71400
E. coli Rosetta 2	EMD Millipore	Cat#71405
E. coli DH5a	Thermo Fisher	Cat#18258-012
Marinomonas mediterranea MMB-1	ATCC	ATCC 700492
Marinomonas mediterranea MMB-1 III-B operonΔ	Andrew Fire	Silas et al. 2017a
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
2216 marine agar	DIFCO	Cat#BD 212185
T4 Polynucleotide Kinase	NEB	Cat#M0201
T4 Polynucleotide Kinase	Epicentre	Cat#P0503K
TurboDNase	Ambion	Cat#AM2238
Phusion High-Fidelity DNA polymerase	Thermo Fisher	Cat#F530L
Phusion PCR master mix	NEB	Cat#M0531L
[γ-32P] ATP (6,000 Ci/mmol)	Perkin-Elmer	Cat#NEG035C005MC
[α-32P] UTP (3,000 Ci/mmol)	Perkin-Elmer	Cat#BLU007H001MC
[α-32P]-dTTP (3,000 Ci/mmol)	Perkin-Elmer	Cat#BLU005H250UC
TGIRT™-III Enzyme	Ingex	Cat#TGIRT10
Critical Commercial Assays
Gibson Assembly Master Mix	NEB	Cat#E2611S
T7 Megascript Kit	Ambion	Cat#AM1334
Oligo Clean and Concentrator kit	Zymo	Cat#D4060
RNA Clean-up and Concentration kit	Zymo	Cat#R1015
Qubit dsDNA assay kit	Life Technologies	Cat#Q32851
MinElute kit	QIAgen	Cat#28004
Ampure XP beads	Beckman-Coulter	Cat#A63881
Deposited Data
MMB-1 Cas6 Fused to MBP	This paper	PDB ID: 6DD5
Figure S2 RNA-seq data	This paper	SRP143510
Figures 5C, 5D, 6A	This paper	SRP142733
Figure 5B	Silas et al., 2017a	SAMN06754958
Figure 5D WT	Silas et al., 2017a	SAMN06754967
Figure 6A WT	Silas et al., 2016	SAMN04259748
Figure S5	Silas et al., 2016	SAMN04259751
Unprocessed and uncompressed imaging data	This paper	DOI: 10.17632/cdpyd694d5.1
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Oligonucleotides
See Table S2 for primer sequences
Recombinant DNA
pMalRF-RTCas1 (WT)	Alan Lambowitz	Silas et al., 2016
pMalRF-RTCas1 Δ307-957	This paper	N/A
pMalRF-RTCas1 H32A	This paper	N/A
pMalRF-RTCas1 H33A	This paper	N/A
pMalRF-RTCas1 H37A	This paper	N/A
pMalRF-RTCas1 R41A	This paper	N/A
pMalRF-RTCas1 S46A	This paper	N/A
pMalRF-RTCas1 S51A	This paper	N/A
pMalRF-RTCas1 S46A S51A (SS)	This paper	N/A
pMalRF-RTCas1 R41A S46A S51A (RSS)	This paper	N/A
pMalRF-RTCas1 H196A, R197A, D200A (HRD)	This paper	N/A
pMalRF-RTCas1 RTΔ	Alan Lambowitz	Silas et al., 2016
pMalRF-RTCas1 E790A	Alan Lambowitz	Silas et al., 2016
pMalRF-RTCas1 E870A	Alan Lambowitz	Silas et al., 2016
pET14b Cas2	Alan Lambowitz	Silas et al., 2016
Plasmid: CRISPR03 only pKT230	Andrew Fire	Silas et al. 2017a
Plasmid: WT CRISPR adaptation pKT230	Andrew Fire	Silas et al. 2016
Plasmid: H32A mutant CRISPR adaptation pKT230	This paper	N/A
Plasmid: H32A-H33A mutant CRISPR adaptation pKT230	This paper	N/A
Plasmid: H37A mutant CRISPR adaptation pKT230	This paper	N/A
Plasmid: R41A mutant CRISPR adaptation pKT230	This paper	N/A
Plasmid: H196A R197A D200A mutant CRISPR adaptation pKT230	This paper	N/A
Plasmid: ∆5-289 mutant CRISPR adaptation pKT230	This paper	N/A
Plasmid: ∆256-289 mutant CRISPR adaptation pKT230	This paper	N/A
Software and Algorithms
XDS	Kabsch, 2010	http://xds.mpimfheidelberg.mpg.de/
Aimless	Evans and Murshudov, 2013	http://www.ccp4.ac.uk/html/aimless.html
PHENIX	Adams et al., 2011	https://www.phenixonline.org/
Coot	Emsley et al., 2010	https://www2.mrclmb.cam.ac.uk/personal/pemsley/coot/
MUSCLE v 3.7	Edgar, 2004	http://www.drive5.com/muscle
HHalign v. 1.5.1	Yu et al., 2015	http://bioserv.rpbs.univ-parisdiderot.fr/services/HHalign-Kbest/
FastTree v 2.1.4	Price et al., 2010	http://www.microbesonline.org/fasttree/
PSIBLAST v. 2.8.0	Altschul et al., 1997	https://www.ncbi.nlm.nih.gov/BLAST/tutorial/Altschul-2.html
UCLUST v1.2.22q	Edgar, 2010	http://www.drive5.com/uclust/downloads1_2_22q.html
Prism v6.0h	N/A	www.graphpad.com
Other
Archaeal and bacterial complete and draft genome sequences (March 2016)	N/A	ftp://ftp.ncbi.nlm.nih.gov/genomes/all/
Cas6 profiles	Makarova et al., 2015	ftp://ftp.ncbi.nlm.nih.gov/pub/wolf/_suppl/CRISPR2015/
CDD profiles	Marchler-Bauer et al., 2015	https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi