REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, Peptides, and Recombinant Proteins | ||
Bathophenantrolinedisulfonic acid disodium salt hydrate (BPS) | Sigma-Aldrich | Cat#146617; CAS 52746-49-3 |
Hygromycin B | Wisent Bioproducts | Cat#450-141-XL; CAS 31282-04-9 |
Metformin-HCl | Jean Coutu | CAS 1115-70-4 |
Methotrexate | BioShop Canada | Cat#MTX440; CAS 59-05-2 |
Nourseothricin-dihydrogen sulfate | Werner Bioagents | Cat#5.005.000; CAS 96736-11-7 |
Rapamycin | BioShop Canada | Cat#RAP004; CAS 53123-88-9 |
Ethidium bromide | BioShop Canada | Cat#ETB444.50; CAS 1239-45-8 |
Carbonyl cyanide m-chlorophenylhydrazone (CCCP) | Sigma-Aldrich | Cat#C2759-250MG; CAS 555-60-2 |
Antimycin A | Sigma-Aldrich | Cat#A8674-25MG; CAS 1397-94-0 |
Oligomycin | Sigma-Aldrich | Cat#495455-10MG; CAS 1404-19-9 |
Acetonitrile | Greyhound | Cat#Bio-012041; CAS 75-05-8 |
Water ULC MS | Greyhound | Cat#Bio-23214102; CAS 7732-18-5 |
Formic acid | Fluka | Cat#O6454; CAS 64-18-6 |
Ammonium acetate | Greyhound | Cat#Bio-012441; CAS 631-61-8 |
Acetic acid | Fluka | Cat#49199-50ml; CAS 64-19-7 |
Ammonium hydroxide | Sigma-Aldrich | Cat#221228-100ml; CAS 1336-21-6 |
dATP/dGTP/dTTP/dCTP | Bioline | Cat#39025 |
Internal standard mix for ICP-MS | Agilent | Cat#5188-6525 |
Multi-element calibration standard-2A | Agilent | Cat#8500-6940 |
Experimental Models: Organisms/Strains | ||
S. cerevisiae: BY4741 | Brachmann et al., 1998 | ATCC 201388 |
S. cerevisiae: mDHFR PCA strain collection | Tarassov et al., 2008 | N/A |
S. cerevisiae: deletion strain collection | Giaever et al., 2002 | N/A |
Recombinant DNA | ||
Plasmid: pHLUM | Mülleder et al., 2012 | N/A |
Plasmid: pYes2-PACT1-pHluorin | Orij et al., 2009 | N/A |
Plasmid: pYes2-PACT1-mtpHluorin | Orij et al., 2009 | N/A |
Software and Algorithms | ||
Fiji (v1.45b) | Schindelin et al., 2012 |
http://imagej.net/Fiji/Downloads; RRID: SCR_002285 |
R (v3.2.3) | The R project |
https://www.r-project.org/; RRID: SCR_001905 |
Cytoscape | Smoot et al., 2011 |
http://cytoscape.org; RRID: SCR_003032 |
limma (R) | Bolstad et al., 2003, Ritchie et al., 2015 |
http://bioconductor.org/packages/release/bioc/html/limma.html; RRID: SCR_010943 |
affy (R) | Bolstad et al., 2003, Gautier et al., 2004 |
http://bioconductor.org/packages/release/bioc/html/affy.html; RRID: SCR_012835 |
cyber-T (R) | Baldi and Long, 2001, Kayala and Baldi, 2012 | N/A |
GOstats (R) | Falcon and Gentleman, 2007 | http://bioconductor.org/packages/release/bioc/html/GOstats.html |
biomaRt (R) | Durinck et al., 2009 | http://bioconductor.org/packages/release/bioc/html/biomaRt.html |
EnrichmentMap (Cytoscape) | Merico et al., 2010 | http://apps.cytoscape.org/apps/enrichmentmap |
AutoAnnotate (Cytoscape) | Kucera et al., 2016 | http://apps.cytoscape.org/apps/autoannotate |
MassHunter | Agilent | https://www.agilent.com/en/products/software-informatics/masshunter-suite/masshunter/masshunter-software; RRID: SCR_015040 |