Figure 6.

STAT-3 Is Required for Inflammation, but Not for T Cell Recruitment or Fibrosis

(A–C) Ptpn2^fl/fl, Alb-Cre;Ptpn2^fl/fl, and Alb-Cre;Ptpn2^fl/fl;Stat-3^fl/+ mice were fed an HFD for 40 weeks. Liver tissue was processed for (A) immunoblotting, (B) real-time PCR, and (C) histology monitoring for immune cell infiltrates and fibrosis (Mason’s Trichrome stain).

(D) The recruitment of liver T cells, including activated effector-memory T cells, was assessed by flow cytometry. Representative and quantified results (means ± SEM) are shown for the indicated number of mice.