Figure S2.

Unaltered Steatosis and Glucose Metabolism In High-Fat-Fed Alb-Cre;Ptpn2^fl/fl Mice, Related to Figure 2

(A–D) Ten-twelve-week-old male liver-specific TCPTP-deficient mice (Alb-Cre;Ptpn2^fl/fl) and Ptpn2^fl/fl littermate controls were fed a HFD for up to 40 weeks. (A) Incremental body weights and (B) body composition at 40 weeks high fat feeding, as assessed by Dual-energy X-ray absorptiometry (DEXA). Livers were extracted from mice fed a HFD for 20 weeks and processed for (C) immunoblot analysis and (D) quantitative (ΔΔCt) real-time PCR to monitor for p-STAT-5 and Igf1 expression respectively.

(E–G) Livers extracted from mice fed a HFD for 40 weeks and processed for (E) histology (Hematoxylin and Eosin), (F) real-time PCR to monitor for fatty acid synthase (FAS; encoded by Fasn), sterol regulatory element-binding protein (SREBP-1c; encoded by Srebf1), stearoyl-CoA desaturase 1 (SCD-1; encoded by Scd1), fatty acid transporter CD36 (Cd36) or peroxisome proliferator-activated receptor γ (PPARγ; encoded by Pparg) and (G) immunoblotting to monitor for steatosis and the expression lipid synthesis genes.

(H–K) Ten-week-old male liver-specific TCPTP-deficient mice (Alb-Cre;Ptpn2^fl/fl) and Ptpn2^fl/fl littermate controls were fed a HFD for 20 weeks. (H) Livers were extracted and processed for analysis of triacylglyceride (TAG), diacylglyceride (DAG) and ceramide content. (I) Mice were fasted for 4-6 h and subjected to insulin (0.75 mU/g) and glucose (2 mg/g) tolerance tests. (J) Fed and fasted (12 h) blood glucose and plasma insulin levels were measured. (K) Ten-twelve week-old male Alb-Cre;Ptpn2^fl/fl and Ptpn2^fl/fl littermate controls were fed a CDAA diet for 12 weeks and body weights and epididymal white adipose tissue (WAT) and liver weights determined. Representative and quantified results (means ± SEM) are shown for the indicated number of mice with significance determined using a Student’s t test.