Figure S4.

The Development of NASH and HCC in High-Fat-Fed Alb-Cre;Ptpn2^fl/fl Mice Is Not Accompanied by Increased PTK and PI3K/AKT/mTOR Signaling, Related to Figure 3

(A–G) Ten-twelve week-old male Alb-Cre;Ptpn2^fl/fl and Ptpn2^fl/fl littermate controls were fed a HFD for 40 weeks and liver tissue and tumors extracted from individual mice for (A)–(E), (G) immunoblot analysis with the indicated antibodies [including those for c-MET, IR β subunit (IRβ), Ser-473 phosphorylated AKT (p-AKT), Thr-180/Tyr-182 phosphorylated p38 (p-p38), Ser-2448 phosphorylated mammalian target of rapamycin (p-mTOR), Thr-37/46 phosphorylated eukaryotic translation initiation factor 4E-binding protein 1 (p-4E-BP1), Ser-235/6 phosphorylated ribosomal protein S6 (p-S6), Tyr-1022/Tyr-1023 phosphorylated JAK-1 (p-JAK-1), Tyr-1007/Tyr-1008 phosphorylated JAK-2 (p-JAK-2), Tyr-418 phosphorylated SFKs (p-SFK)], or (F) real time PCR. Representative and quantified results (means ± SEM) are shown for the indicated number of mice with significance determined using a Student’s t test.