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. 2018 Nov 2;7:e39800. doi: 10.7554/eLife.39800

Figure 2. Generation of MSP121-specific AMB in response to mosquito transmitted P.chabaudi infection.

Figure 2.

(A) Flow cytometry showing differential expression of CD11b and CD11c on splenic MSP121-specific B cells from NIMP23→Rag2-/- chimeric mice before infection (day 0) and at 35 and 155dpi. (B) Numbers of splenic MSP121-specific CD11b+CD11c+ AMB from NIMP23→Rag2-/- during the course of mosquito transmitted P. chabaudi infection. Kruskal-Wallis test vs day 0. ****, p<0.0001 (C) Flow cytometry showing expression of CD21/35, FCRL5, IgD, CD273 and CD80 on different subsets of splenic MSP121-specific B cells from NIMP23→Rag2-/- chimeric mice defined based on CD11b and CD11c expression at 35dpi. (D) Geometric mean fluorescence intensity (MFI) of CD21/35, FCRL5, IgD, CD273 and CD80 expression on different subsets of splenic MSP121-specific B cells from NIMP23→Rag2-/- chimeric mice defined based on CD11b and CD11c expression at 35dpi. (E) Frequencies of CD21/35, FCRL5, IgD, CD273 and CD80 positive cells among different subsets of splenic MSP121-specific B cells from NIMP23→Rag2-/- chimeric mice defined based on CD11b and CD11c expression at 35dpi. Two-way ANOVA vs CD11b-CD11c- subset. *p<0.05; **p<0.01, ***p<0.001; ****p<0.0001. Error bars are SEM. Data pooled from three independent experiments with 3–5 mice per group.