(A) Principal component analysis of RNAseq transcriptome data from splenic MSP121-specific AMB (CD11b+CD11c+, 35dpi), CD11b—CD11c— B cells (35dpi), Bmem (FCRL5hi, 155dpi), FCRL5— B cells (155dpi) and B cells from naïve mice (0dpi). The MSP121-specific AMB and Bmem are contained inside ellipses. (B) (C) (D) (E) Normalized counts corresponding to selected genes representing memory B-cell markers, anti and pro-apoptotic genes, immunoglobulins and atypical memory B-cell markers, respectively, for all five groups described in (A). Each bar represents an individual mouse. Data generated with five mice per group.