Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
B6.SJL-Ptprca Pepcb/BoyJ (B6.CD45.1) |
The Jackson Laboratory |
MGI:4819849 | Bred in the specific pathogen-free facilities of the MRC National Institute for Medical Research and The Francis Crick Institute |
| Genetic reagent (M. musculus) |
Rag2tm1Fwa(Rag2-/-) | The Jackson Laboratory |
MGI:1858556 | Bred in the specific pathogen- free facilities of the MRC National Institute for Medical Research and The Francis Crick Institute |
| Genetic reagent (M. musculus) |
IghNIMP23/+ | This paper | _ | Bred in the specific pathogen-free facilities of the MRC National Institute for Medical Research and The Francis Crick Institute |
| Strain, strain background (Plasmodium chabaudi chabaudi, strain AS) |
P. chabaudi | other | _ | European Malaria Reagent Repository, University of Edinburgh. |
| Strain, strain background (Anopheles stephensi, strain SD500, female) |
mosquitos | PMID: 23217144 | _ | Bred in Jean Langhorne's lab |
| Antibody | Monoclonal Rat Anti-CD11b |
BD Biosciences | 563553 | (dil 1/50) |
| Antibody | Monoclonal Hamster Anti-Mouse CD11c |
BD Biosciences | 561022 | (dil 1/50) |
| Antibody | Monoclonal Rat Anti-Mouse CD138 |
BD Biosciences | 553714 | (dil 1/400) |
| Antibody | Monoclonal Rat Anti-Mouse CD19 |
BD Biosciences | 565076 | (dil 1/200) |
| Antibody | Monoclonal Rat anti-Mouse CD19 |
Biolegend | 115530 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD19 |
Biolegend | 115543 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD1d |
Biolegend | 123510 | (dil 1/100) |
| Antibody | Monoclonal Rat anti-mouse CD2 |
Biolegend | 100112 | (dil 1/100) |
| Antibody | Monoclona Rat Anti-Mouse CD21/35 |
BD Biosciences | 563176 | (dil 1/100) |
| Antibody | Monoclona Rat Anti-Mouse CD21/35 |
BD Biosciences | 553818 | (dil 1/100) |
| Antibody | Monoclonal Rat anti-Mouse CD23 |
eBioscience | 25–0232 | (dil 1/100) |
| Antibody | Monoclonal Rat anti-Mouse CD273 |
BD Biosciences | 564245 | (dil 1/25) |
| Antibody | Armenian Hamster anti-Mouse CD3 |
Biolegend | 100336 | (dil 1/100) |
| Antibody | Monoclonal Rat anti-Mouse CD38 |
Biolegend | 102718 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD38 |
eBioscience | 17–0381 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD38 |
BD Biosciences | 740697 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD4 |
Biolegend | 100414 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD45.1 |
Biolegend | 110706 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD45.1 |
Biolegend | 110728 | (dil 1/400) |
| Antibody | Monoclonal Mouse anti-Mouse CD45.2 |
BD Biosciences | 563685 | (dil 1/50) |
| Antibody | Monoclonal Mouse anti-Mouse CD45.2 |
Biolegend | 109814 | (dil 1/50) |
| Antibody | Monoclonal Mouse anti-Mouse CD45.2 |
Biolegend | 109808 | (dil 1/50) |
| Antibody | Monoclonal Rat anti-Mouse CD45R/B220 |
BD Biosciences | 564449 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD45R/B220 |
Biolegend | 103224 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD45R/B220 |
eBioscience | 25–0452 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD73 |
BD Biosciences | 550741 | (dil 1/100) |
| Antibody | Monoclonal Armenian Hamster anti-Mouse CD80 |
Biolegend | 104729 | (dil 1/25) |
| Antibody | Monoclonal Rat anti-Mouse CD8a |
Biolegend | 100734 | (dil 1/400) |
| Antibody | Monoclonal Rat anti-Mouse CD9 |
BD Biosciences | 558749 | (dil 1/100) |
| Antibody | Monoclonal Rat anti-Mouse CD93 (AA4.1) |
eBioscience | 17–5892 | (dil 1/100) |
| Antibody | FCRL5 | PMID: 17082595 | _ | Produced in Randall Davis' lab (dil 1/400) |
| Antibody | Polyclonal Sheep anti-Mouse FCRL5 |
R and D Systems | FAB6756G | (dil 1/50) |
| Antibody | Monoclonal Rat Anti-Mouse T- and B-Cell Activation Antigen GL7 |
BD Biosciences | 562080 | (dil 1/100) |
| Antibody | Monoclonal Rat Anti-Mouse IgD |
Biolegend | 405725 | (dil 1/400) |
| Antibody | Monoclonal Rat Anti-Mouse IgD |
Biolegend | 405723 | (dil 1/400) |
| Antibody | Monoclonal Rat Anti-Mouse IgD |
Biolegend | 405710 | (dil 1/400) |
| Antibody | Monoclonal Rat Anti-Mouse IgG2b |
Biolegend | 406708 | (dil 1/25) |
| Antibody | Monoclonal Rat Anti-Mouse IgM |
Biolegend | 406512 | (dil 1/100) |
| Recombinant DNA reagent | VDJHNIMP23
anti-MSP121 variable region coding exon containing the Leader-V segment intron from gDNA of the NIMP23 hybridoma |
PMID: 7141700 | _ | Produced in Jean Langhorne's lab |
| Recombinant DNA reagent | C57Bl/6 IgH HEL variable region knock-in construct |
PMID: 12668643 | _ | Donated by Robert Brink of the Garvan Institute of Medical Research, New South Wales, Australia |
| Peptide, recombinant protein | MSP121 | PMID: 11254580 | _ | Produced in Jean Langhorne's lab |
| Commercial assay or kit | EZ-Link Sulfo-NHS-LC-Biotinylation Kit |
Thermo Scientific | 21435 | |
| Commercial assay or kit | RiboPure RNA Purification Kit |
Invitrogen | AM1924 | |
| Commercial assay or kit | Qubit 1X dsDNA HS Assay Kit |
Invitrogen | Q33231 | |
| Commercial assay or kit | SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing |
Takara | 634889 | |
| Commercial assay or kit | Ovation Ultralow Library System V2 |
Nugen | 0344–32 | |
| Commercial assay or kit | LIVE/DEAD Fixable Aqua Dead Cell Stain Kit |
Invitrogen | L34957 | |
| Commercial assay or kit | LIVE/DEAD Fixable Blue Dead Cell Stain Kit |
Invitrogen | L23105 | |
| Chemical compound, drug | Streptavidin-R-Phycoerythrin | Prozyme | PJRS25 | |
| Chemical compound, drug | Streptavidin-Allophycocyanin | Prozyme | PJ27S | |
| Chemical compound, drug | TiterMax Gold Adjuvant |
Merck (formerly Sigma-Aldrich) | T2684-1ML | |
| Chemical compound, drug | R848 (Resiquimod) | Invivogen | tlrl-r848 | |
| Chemical compound, drug | TRI Reagent Solution |
Invitrogen | AM9738 | |
| Software, algorithm | cutadapt v1.9.1 | doi:10.14806/ej.17.1.200 | ||
| Software, algorithm | RSEM v1.2.31 | doi:10.1186/1471-2105-12-323 | ||
| Software, algorithm | STAR v2.5.1b | doi:10.1093/ bioinformatics/bts635 |
||
| Software, algorithm | DESeq2 | doi:10.1186/s13059-014-0550-8 | ||
| Software, algorithm | R v3.4.0 | other | https://www.r-project.org | |
| Software, algorithm | Bioconductor v3.5 |
other |
http://www.
bioconductor.org |
|
| Software, algorithm | Broad's Gene Set Enrichment Analysis (GSEA) |
other | http://software.broadinstitute.org/gsea/index.jsp | |
| Software, algorithm | FlowJo version 9.6 or higher | Tree Star | ||
| Software, algorithm | Cytofkit | doi:10.1371/journa l.pcbi.1005112.s009 |
||
| Software, algorithm | Prism v6 | GraphPad |