Fig 3. Pro-σ^K-CFP localization to the membranes surrounding the forespore requires the B protease.

Representative images of the indicated strains at hour 4 of sporulation. Pro-σ^K-CFP localizes to the membranes surrounding the forespore in cells harboring B(E44Q)-YFP, in the absence of A, BofA, or both. In sporulating cells lacking the B protease, pro-σ^K localizes to the mother-cell cytoplasm with some enrichment on the nucleoid. The cytoplasmic pro-σ^K-CFP signal is more pixelated in the strains lacking A and/or BofA compared to the ΔB mutant (see text). Images were scaled identically. Scale bar indicates 2 μm. Quantification of forespore-associated pro-σ^K-CFP and larger fields of cells for all 5 strains over a sporulation time course can be found in S4 and S5 Figs.