Figure 3.
The N Terminus of UHRF1 Is Required for Targeted Ubiquitin Transfer from the E2 to Histone H3
(A) E3 assays using 12 × 187 bp chromatin arrays as substrate. Deletion of Met-Trp at the very N terminus substantially decreases UHRF1-mediated H3 ubiquitylation while increasing UHRF1 auto-ubiquitylation as detected by higher molecular weight species in the anti-HA blot.
(B) UHRF1ΔMW auto-ubiquitylation and H3 ubiquitylation is stimulated in the presence of 12 × 187 bp arrays in E3 assays when compared against mono-, di-, and tetra-nucleosomes, where very little E3 activity is detected.