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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Allergy. 2018 Jul 26;73(11):2251–2254. doi: 10.1111/all.13552

Figure 2. IL-33 was not elevated in NPs.

Figure 2.

Expression of mRNAs for full-length IL-33 (A), IL-33∆exon3,4 (B) and IL-33∆exon3,4s5 (C) was determined by RNase H-dependent quantitative RT-PCR in control ethmoid sinus tissue (Control; n = 34) and NPs from CRSwNP (n = 46) and AERD (n = 23). IL-33 protein in tissue extracts was determined by Luminex (D, control (n = 26), CRSwNP NP (n = 37) and AERD NP (n = 13)) and Western blot (E). IL-33 protein concentration was normalized to the concentration of total protein (D). The results are representative of 2 separate experiments with separate donors (control (n = 12) and NP (n = 11)) (E). Representative immunostaining for IL-33 is shown in a control ethmoid tissue and an NP tissue (F). Magnification; ×400 (upper) and ×1200 (lower). Results are shown as median with interquartile range.