Figure 6.

ToxR transcriptional control of ompT and ompU is dependent on ToxS under DC activating conditions. Shown are reporter gene activities of alkaline phosphatase PhoA (Miller Units) linked as operon fusions to either ompU (A) or ompT (B) in V. cholerae WT, toxS, FLAGtoxR^CC and toxR strains. Simultaneously, immunoblot analyses were performed utilizing α‐ToxR antiserum. Strains were grown in M9 maltose in the presence (dark bars) or absence (open bars) of 0.01% DC until OD₆₀₀ of 0.8‐1. Data shown are mean and standard deviation of six independent samples for each condition. The asterisks indicate a statistically significant difference between bile salt‐treated and non‐treated cells, with P‐values by paired t‐test (*): P < 0.05. (•): Represents a nonspecific cross‐reacting background band.