Table 2.
Oligonucleotidesa (5′‐3′) used in this study.
| XbaI_dtoxS_fwd | TTTTCTAGATGGATTATTCTAAGTCTGCAT | |
| SacI_dtoxS _rev | ATTGAGCTCCCATGAAACTATTTTTTGTCTC | |
| SOE_dtoxS_rev | TCAGTCAGGAGCAAGATCCTACTCACACACTTTGAT | |
| SOE_dtoxS_fwd | GATCTTGCTCCTGACTGAGCGTAGAATAGGACATAA | |
| HindIII_toxRCC_dtoxS_rev | TTTAAGCTTAGCAAGATCCTACTCAGACA | |
| HindIII_dtoxS_fwd | TATAAGCTTCCTGACTGAGCGTAGAATA | |
| NcoI_cat_fwd | TTTACCGGTCAACCAGGCGTTTAAGGG | |
| NcoI_cat_rev | TTTACCGGTTTCCAACTTTCACCATAATGA | |
| SacI_ddegS _fwd | AAAGAGCTCAAAAATCCCCAAACTTGCA | |
| NcoI_ddegS_rev | TAAACCGGTTATAAATATTGACGACGGCA | |
| NcoI_ddegS_fwd | TTTACCGGTCGCCAGAATGTCACAGATA | |
| XbaI_ddegS_rev | ATATCTAGAGAAGCTGGCTAAGAAGTAATGCT | |
| SacI_ompU_fwd | TTTGAGCTCCTTTAATAGTCTATCGAGTTCTT | |
| SthI_ompU_rev | TTTTGGTACCGAAGTACCTTTCGCGCT | |
| SacI_ompT_fwd | ATGAGCTCGGTGATTTGGCTGTGCACG | |
| SthI_ompT_rev | TAGGTACCTTACCAGTAGATACGAGCACC | |
| XhoI_rseA_fwd | TAACTCGAGACTATGGTGAATAGAATGGC | |
| KpnI_rseC_rev | ATTGGTACCTTTCGTTAGGGTTCGCATCA | |
| template for pelBYYF | GCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCCACCACCACCACCACCACTCAGCAGATAATTTTGCTATCGACGCAACTTACTACTTCAAGCCAAACTTCCGCTCTTACATCTCTTACCAGTTCAATCTGCTAGATTCAGACAAAGTTGGTAAAGTAGCATCAGAAGACGAACTGGCTA | |
| NcoI_tripep_fwd | ATTCCATGGGAAAATACCTGCTGCCGACCGCTGCTGCTGGTCT | |
| Pst_YYF_tripep_rev | AATCTGCAGTTAGAAGTAGTAACGTAGACCGA TAGCCAGTTCGTCTTCTGATG | |
| bla_inv_rev | CCGTAAGATGCTTTTCTGTGACTGGT | |
| ompU_seq_fwd | CCAACAAACATTAAAATCATTTAA | |
| pFLAGMAC_fwd | AACGGTTCTGGCAAATATTC | |
| pTricHis_rev | CTTCTGCGTTCTGATTTAATCTG | |
| rseB_seq_fwd | GACTCGTGATTCGGTGGA | |
| M13rev‐48_fwd | AGCGGATAACAATTTCAC | |
| SphI_degP_fwd | TTAGCATGCAGGTCGTACCACTGGCT | |
| HindIII_degP_rev | AAAAAGCTTAACGTCCGGTTGAAGT | |
| SacI_depP_phoA‐fusion_fwd | TTAGAGCTCCGGTGGCAACGTCGGTAT | |
| KpnI_degP_phoA‐fusion_rev | TTAGGTACCTTAACGAACAACCAAGTAAAGCG | |
| EcoRI_VC0566_compl_fwd | TTTGAATTCTTTGTTGAGGAGCTTATGATG | |
| BamHI_VC0566_compl_rev | TATGGATCCTTAACGAACAACCAAGTAAAGC | |
| BamHI_degS_fwd | TTTGGATCCTCGCCAGAATGTCACAGAT | |
| SmaI_degS_rev | TTTCCCGGGTGACTGACGAACAGGAAC | |
| SacI_degS_fwd | ATAGAGCTCAAAAATCCCCAAACTTGC | |
| XbaI_degS_rev | ATATCTAGAAGCTGGCTAAGAAGTAAT | |
| HindIII_cat_fwd | ATAAAGCTT AGCACCTCAAAAACACCATC | |
| BamHI_cat_rev | TTAGGATCC CACCAGGCGTTTAAGGGCA | |
| M13_pUC_rev | AGCGGATAACAATTTCACACAGG | |
| pGP704_CVD_rv|15 | GATGTAACGCACTGAGAAG | |
a
Restriction sites are underlined