Table 3.

qRT-PCR Cq values with standard deviation for mixtures at different sample weight ratios.

Detected species	Sample composition of mixture	Cq values with standard deviation of different sample weight ratios
		10:1	50:1	100:1	200:1	500:1	1000:1	2000:1	5000:1
Cy. songaricum	Ci. deserticola: Cy. songaricum	24.92 ± 0.188	27.1 ± 0.022	27.81 ± 0.147	29.24 ± 0.394	30.47 ± 0.304	31.39 ± 0.561	31.42 ± 0.406	32.46 ± 0.862
Ci. sinensis	Ci. deserticola: Ci. sinensis	23.64 ± 0.078	26.00 ± 0.101	27.07 ± 0.155	28.26 ± 0.109	29.46 ± 0.066	30.47 ± 0.125	31.36 ± 0.101	33.36 ± 0.190
B. rossica	Ci. deserticola: B. rossica	18.27 ± 0.138	21.32 ± 0.821	22.28 ± 0.033	25.78 ± 0.020	26.25 ± 0.031	27.22 ± 0.052	29.33 ± 0.823	30.45 ± 0.262
O. coerulescens	Ci. deserticola: O. coerulescens	19.07 ± 0.022	21.34 ± 0.030	22.53 ± 0.105	23.77 ± 0.111	25.16 ± 0.089	26.36 ± 0.098	27.30 ± 0.164	29.25 ± 0.049
Ci. tubulosa	Ci. deserticola: Ci. tubulosa	24.05 ± 0.145	26.93 ± 0.176	27.97 ± 0.044	29.56 ± 0.161	30.60 ± 0.338	31.18 ± 0.090	32.25 ± 0.122	33.70 ± 0.222
Ci. deserticola	Ci. tubulosa: Ci. deserticola	23.93 ± 0.165	25.06 ± 0.155	26.25 ± 0.093	28.05 ± 0.085	29.07 ± 0.163	30.04 ± 0.194	31.82 ± 0.107	32.90 ± 0.424
		10000:1	15000:1	20000:1	25000:1	30000:1	40000:1	50000:1	60000:1
Cy. songaricum	Ci. deserticola: Cy. songaricum	34.48 ± 0.255	35.83 ± 0.328	None	None	None	None	None	None
Ci. sinensis	Ci. deserticola: Ci. sinensis	34.33 ± 0.214	35.45 ± 0.125	36.26 ± 0.756	None	None	None	None	None
B. rossica	Ci. deserticola: B. rossica	31.98 ± 0.804	32.79 ± 0.361	33.80 ± 0.550	35.46 ± 0.886	37.08 ± 0.162	None	None	None
O. coerulescens	Ci. deserticola: O. coerulescens	30.01 ± 0.260	31.36 ± 0.284	32.84 ± 0.338	33.52 ± 0.215	36.38 ± 0.459	36.16 ± 0.509	None	None
Ci. tubulosa	Ci. deserticola: Ci. tubulosa	34.90 ± 0.603	35.95 ± 0.470	36.48 ± 0.501	None	None	None	None	None
Ci. deserticola	Ci. tubulosa: Ci. deserticola	35.17 ± 0.183	35.23 ± 0.544	36.25 ± 0.279	None	None	None	None	None