Table 4.

Primers used for PCR amplification and sequencing.

Primer pair	Primer name	Direction	Primer sequences (5′-3′)	Amplicon size	Target species	Thermal program
1	SYF1	Forward	CTCAATGTGCTGCTGCTT	123	Cy. songaricum	94°C for 2 min;
	SYR1	Reverse	AGACTTACCGCTCACAATG			98°C for 10 s,
2	HMRCF	Forward	CCTTTAGGGTGATACTTAGGT	132	Ci. deserticola	50°C for 30 s,
	HMRCR	Reverse	CAGCACGAGAGTTGAGAG			68°C for 15 s, 35 cycles;
3	GHRCF	Forward	TTCTGGGACAATGCTTAGG	134	Ci. tubulosa
	GHRCR	Reverse	CGACACGAGAGTTGAGTT
4	SCRF	Forward	ATATGGGCGATAGGTAGGT	131	Ci. sinensis
	SCRR	Reverse	GACAGCACGAGAGTTGAG
5	CCRF	Forward	CGGTCCAAATACGATCCC	72	B. rossica
	CCRR	Reverse	GACAGCACGAGAGTTGAG
6	LDF	Forward	ATCTTCAACTCTCGTCTGTC	71	O. coerulescens
	LDR	Reverse	CTCGTGCCTATGGGTCTA