Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 19;9:4559. doi: 10.1038/s41467-018-07077-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — G9a/Glp inhibition drives cells towards the TPC phenotype. a Scatter plot of fold change in median fluorescence of drug-screened CK1750 cells labeled with fluorescent anti-CD24 and anti-Sca-1 antibodies. Each point represents an individual compound, red = UNC0638, blue = other compounds. b Representative (n = 5) FACS histogram of Sca-1 fluorescence of lung adenocarcinoma cells following 96 h. treatment with 1 µM UNC0638 or vehicle control, gated for live single cells. c qPCR of Sca-1 mRNA normalized to Gapdh from adenocarcinoma cells following 96 h. treatment with 1 µM UNC0638 or vehicle control. Error bars denote standard deviation. *P < 0.05, t test, n = 3. d Bar chart of % of cells forming organoids 10 days after 1000 adenocarcinoma cells were plated in 3D culture with 1 µM UNC0638 or DMSO following 96 h. pre-treatment with UNC0638 or DMSO control. Error bars denote standard deviation. *P < 0.05, t test, n = 3. e Representative bar chart of % of cells forming organoids 10 days after 5000 single cells isolated from a primary adenocarcinoma were plated in 3D culture with 1 µM UNC0638 or DMSO. Error bars denote standard deviation. *P < 0.05, t test, n = 3 technical replicates. f Experimental design of G9ai in intravenous transplantation assay, and table of tumor incidence in recipients of UNC0638-treated or DMSO-treated adenocarcinoma cells by intravenous injection. *P < 0.05, Fisher’s exact test. g Bar chart of mRNA abundance of G9a and Glp normalized to β-Actin in Sca-1+ cells (TPCs) relative to Sca-1− cells (non-TPCs) from FACS-sorted primary adenocarcinomas, gated for single, live, CD31−, CD45− cells. Error bars denote standard deviation. *P < 0.05, t test, n = 3. h Representative (n = 3) images of isolated lung adenocarcinoma cells sorted by CD24 and Sca-1, gated for live, single, CD31−, CD45−, Epcam+ cells after cytospin and immunostained for the indicated markers. Scale bar = 20 µm. i Quantification of H3K9me2 and Sca-1 immunostaining of isolated lung adenocarcinoma cells sorted by CD24 and Sca-1, gated for live, single CD31−, CD45−, Epcam+ cells. Error bars denote standard deviation. *P < 0.05, t test, n = 3