Fig. 2.

G9a depletion expands the TPC population and promotes tumor progression and metastasis. a Cartoon of experimental design to deplete G9a in Cre-induced adenocarcinomas. b Box and Whisker plot of tumor number per mouse, in recipients of control and shG9a lentiviruses. Boxes represent inner quartiles, center line = median, whiskers = 1.5 × IQR. *P < 0.05, Mann–Whitney test. c Bar chart of tumor grade/mouse of control and shG9a recipients. *P < 0.05, χ² test, n = 16 and n = 24 mice, respectively. d Table of metastasis occurrence in the recipients of control and shG9a lentiviruses. *P < 0.05, Fisher’s exact test. e Representative FACS plots of dissociated tumors from control (n = 9) and shG9a (n = 11) recipients, gated for live, single, CD31−, CD45−, Epcam+ cells. f Chart showing the median proportion of CD24+Sca-1+ TPCs in control and shG9a tumors determined by FACS. Each data point represents tumors from an individual mouse. *P < 0.05, Mann–Whitney test. g Table of secondary tumor and metastasis incidence and location in recipients of orthotopically transplanted control and shG9a tumor cells. *P < 0.05, Fisher’s exact test. h Table of tumor incidence and TPC frequency in recipients of control or G9ai adenocarcinoma cells in limiting dilution assay. TPC frequency and P value (χ²) were calculated by ELDA software