Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 19;8:17027. doi: 10.1038/s41598-018-35391-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunofluorescence analysis for the protein expression of mitochondrial biomarkers by tenocytes cultured under (A) hypoxic and (B) normoxic conditions: Images in the left columns show nuclear staining with DAPI; the images in the middle column show expression of mitochondrial biomarkers while the images in the right column show overlay of mitochondrial biomarkers with DAPI. Images were acquired at 20x magnification using CCD camera attached to the Olympus microscope. (C) The image shows quantification of the expression mitochondrial biomarkers. The intensity of protein expression as observed through immunofluorescence was acquired and the mean fluorescence intensity (MFI) was quantified from 30 cells of each groups. The graphs represent MFI mean values with standard error. The statistical significance of each hypoxic groups vs normoxic groups are represented in the figure (n = 30; NS – non-significant, *P < 0.05, **P < 0.01 and ***P < 0.001).