Fig. 2.

Overexpression of MDA5 or RIG-I restricts KSHV lytic reactivation in iSLK.219 cells. a iSLK.219 F-RIG-I, F-MDA5, and Control (Con) cells were reactivated for 72 h at which time GFP and RFP images were captured. Bar indicates 750 μm. b RNA was extracted from cells in (a) and expression of the indicated viral genes was quantified by RT-qPCR. c Western blot analysis of cell lysates prepared from cells described in (a). d HEK293T cells were infected with supernatants from cells described in (a). GFP images were taken 48 h postinfection. Bar indicates 300 μm. e Quantification of GFP-positive cells in (d). f RNA was extracted from HEK293T cells in (d). Expression of KSHV LANA was quantified by RT-qPCR. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, ^*p < 0.05, ^**p < 0.01