Fig. 3.

RLRs-MAVS signaling pathway restricts KSHV lytic reactivation in BC-3 cells. a RNA was extracted from BC-3 cells expressing the indicated shRNAs and the target mRNA level was quantified by RT-qPCR. b Expression of KSHV viral genes in cells described in (a) was quantified by RT- qPCR. c BC-3 F-RIG-I, F-MDA5, and Control (Con) cells were reactivated for 48 h. Cell lysates from latent and lytic cells were analyzed by immunoblotting for the indicated proteins. d RNA was extracted from BC-3 cells described in (c) and expression of the indicated viral genes was quantified by RT-qPCR. Error bars in all panels represent mean ± SD from three independent experiments. p-values were determined by the Student’s t test, ^*p < 0.05, ^**p < 0.01