Fig. 4.

RIG-I and MDA5 bind host RNAs during lytic reactivation in PEL. a HCT116 dual cells were transfected with RNA purified from latent and lytic BC-3 cells either by IgG or J2 antibody. Cells were harvested 24 h posttransfection and subjected to luciferase assay. Mock indicates no antibody. b Pie chart representation of gene biotypes identified by F-RIG-I and F-MDA5 fRIP-seq. c PCA analysis. d, e The gene ontology overrepresentation analysis of enriched RNAs from F-MDA5 (d) and F-RIG-I (e) fRIP-seq. f Distribution of MDA5 fRIP-seq reads on the NOP14 locus. g fRIP-qPCR analysis of NOP14 and GINS. h Distribution RIG-I fRIP-seq reads at the vtRNA loci. i fRIP-qPCR analysis of vtRNAs. Error bars in all panels represent mean ± SD from three independent experiments. p Values were determined by the Student’s t test, ^*p < 0.05, ^**p < 0.01