Fig. 7. The effect of 3-MA on proliferation and apoptosis of RACK1 OE colon cancer cells.

a Western blot analysis showing the expression levels of LC3-II in the SW480-RACK1 OE cells treated with 3-methyladenine (3-MA). Analysis of cell proliferation by CCK-8 (b), plate clone formation (c) and EdU incorporation (d) assay in the SW480-RACK1 OE cells treated with 3-MA and control cells. Analysis of cell cycle distribution (e) and cell apoptosis (f) by flow cytometry in the SW480-RACK1 OE cells treated with 3-MA and control cells. Three experiments were done; Means, SDs, and statistical significance are denoted; *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance. Scale bars = 200 μm